Please use this identifier to cite or link to this item:
Title: Polymerase III transcription is necessary for T cell priming by dendritic cells
Author: Reverendo, Marisa
Argüello, Rafael J.
Polte, Christine
Valecka, Jan
Camosseto, Voahirana
Auphan-Anezin, Nathalie
Ignatova, Zoya
Gatti, Evelina
Pierre, Philippe
Keywords: CD86
Casein kinase 2
Protein synthesis
Issue Date: 5-Nov-2019
Publisher: National Academy of Sciences
Abstract: Exposure to microbe-associated molecular patterns (MAMPs) causes dendritic cells (DCs) to undergo a remarkable activation process characterized by changes in key biochemical mechanisms. These enhance antigen processing and presentation, as well as strengthen DC capacity to stimulate naïve T cell proliferation. Here, we show that in response to the MAMPS lipopolysaccharide and polyriboinosinic:polyribocytidylic acid (Poly I:C), RNA polymerase III (Pol lII)-dependent transcription and consequently tRNA gene expression are strongly induced in DCs. This is in part caused by the phosphorylation and nuclear export of MAF1 homolog negative regulator of Poll III (MAF1), via a synergistic casein kinase 2 (CK2)- and mammalian target of rapamycin-dependent signaling cascade downstream of Toll-like receptors (TLRs). De novo tRNA expression is necessary to augment protein synthesis and compensate for tRNA degradation driven by TLR-dependent DC exposure to type-I IFN. Although protein synthesis is not strongly inhibited in absence of RNA Pol III activity, it compromises the translation of key DC mRNAs, like those coding for costimulatory molecules and proinflammatory cytokines, which instead can be stored in stress granules, as shown for CD86 mRNA. TLR-dependent CK2 stimulation and subsequent RNA Pol III activation are therefore key for the acquisition by DCs of their unique T cell immune-stimulatory functions.
Peer review: yes
DOI: 10.1073/pnas.1904396116
ISSN: 0027-8424
Appears in Collections:IBIMED - Artigos
DCM - Artigos

Files in This Item:
File Description SizeFormat 
Pierre PNAS2019 PolIII transciption is necessary for T cell priming by DC.pdf2.21 MBAdobe PDFView/Open

Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.