Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/26735
Title: Excreted thiocyanate detects live reef fishes illegally collected using cyanide: a non-invasive and non-destructive testing approach
Author: Vaz, Marcela C. M.
Rocha-Santos, Teresa A. P.
Rocha, Rui J. M.
Lopes, Isabel
Pereira, Ruth
Duarte, Armando C.
Rubec, Peter J.
Calado, Ricardo
Issue Date: 2012
Publisher: Public Library of Science
Abstract: Cyanide fishing is a method employed to capture marine fish alive on coral reefs. They are shipped to markets for human consumption in Southeast Asia, as well as to supply the marine aquarium trade worldwide. Although several techniques can be used to detect cyanide in reef fish, there is still no testing method that can be used to survey the whole supply chain. Most methods for cyanide detection are time-consuming and require the sacrifice of the sampled fish. Thiocyanate anion (SCN(-)) is a metabolite produced by the main metabolic pathway for cyanide anion (CN(-)) detoxification. Our study employed an optical fiber (OF) methodology (analytical time <6 min) to detect SCN(-) in a non-invasive and non-destructive manner. Our OF methodology is able to detect trace levels (>3.16 µg L(-1)) of SCN(-) in seawater. Given that marine fish exposed to cyanide excrete SCN(-) in the urine, elevated levels of SCN(-) present in the seawater holding live reef fish indicate that the surveyed specimens were likely exposed to cyanide. In our study, captive-bred clownfish (Amphiprion clarkii) pulse exposed for 60 s to either 12.5 or 25 mg L(-1) of CN(-) excreted up to 6.96±0.03 and 9.84±0.03 µg L(-1) of SCN(-), respectively, during the 28 days following exposure. No detectable levels of SCN(-) were recorded in the water holding control organisms not exposed to CN(-), or in synthetic seawater lacking fish. While further research is necessary, our methodology can allow a rapid detection of SCN(-) in the holding water and can be used as a screening tool to indicate if live reef fish were collected with cyanide.
Peer review: yes
URI: http://hdl.handle.net/10773/26735
DOI: 10.1371/journal.pone.0035355
Appears in Collections:CESAM - Artigos
DBio - Artigos
DQ - Artigos

Files in This Item:
File Description SizeFormat 
Vaz et al. - 2012 - Excreted Thiocyanate Detects Live Reef Fishes Ille.pdf337.64 kBAdobe PDFView/Open


FacebookTwitterLinkedIn
Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.