Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/26712
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dc.contributor.authorSoares, Ana Raquelpt_PT
dc.contributor.authorPereira, Patrícia M.pt_PT
dc.contributor.authorFerreira, Violetapt_PT
dc.contributor.authorReverendo, Marisapt_PT
dc.contributor.authorSimões, Joãopt_PT
dc.contributor.authorBezerra, Ana Ritapt_PT
dc.contributor.authorMoura, Gabriela R.pt_PT
dc.contributor.authorSantos, Manuel A. S.pt_PT
dc.date.accessioned2019-10-09T14:28:12Z-
dc.date.available2019-10-09T14:28:12Z-
dc.date.issued2012-05-
dc.identifier.issn1096-6080pt_PT
dc.identifier.urihttp://hdl.handle.net/10773/26712-
dc.description.abstractPrenatal exposure to ethanol leads to a myriad of developmental disorders known as fetal alcohol spectrum disorder, often characterized by growth and mental retardation, central nervous system damage, and specific craniofacial dysmorphic features. The mechanisms of ethanol toxicity are not fully understood, but exposure during development affects the expression of several genes involved in cell cycle control, apoptosis, and transcriptional regulation. MicroRNAs (miRNAs) are implicated in some of these processes, however, it is not yet clear if they are involved in ethanol-induced toxicity. In order to clarify this question, we have exposed zebrafish embryos to ethanol and evaluated whether a miRNA deregulation signature could be obtained. Zebrafish embryos were exposed to 1 and 1.5% of ethanol from 4 h postfertilization (hpf) to 24 hpf. The miRNA expression profiles obtained reveal significant miRNA deregulation and show that both ethanol concentrations upregulate miR-153a, miR-725, miR-30d, let-7k, miR-100, miR-738, and miR-732. Putative gene targets of deregulated miRNAs are involved in cell cycle control, apoptosis, and transcription, which are the main processes affected by ethanol toxicity. The conservation of affected mechanisms among vertebrates leads us to postulate that similar miRNA deregulation occurs in humans, highlighting a relevant role of miRNAs in ethanol toxicology.pt_PT
dc.language.isoengpt_PT
dc.publisherOxford University Presspt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F32945%2F2006/PTpt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/64745/PTpt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/72251/PTpt_PT
dc.rightsrestrictedAccesspt_PT
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEthanol toxicitypt_PT
dc.subjectMicroRNAspt_PT
dc.subjectZebrafishpt_PT
dc.subjectApoptosispt_PT
dc.titleEthanol exposure induces upregulation of specific microRNAs in zebrafish embryospt_PT
dc.typearticlept_PT
dc.description.versionpublishedpt_PT
dc.peerreviewedyespt_PT
degois.publication.firstPage18pt_PT
degois.publication.issue1pt_PT
degois.publication.lastPage28pt_PT
degois.publication.titleToxicological Sciencespt_PT
degois.publication.volume127pt_PT
dc.identifier.doi10.1093/toxsci/kfs068pt_PT
dc.identifier.essn1096-0929pt_PT
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