Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/26712
Title: Ethanol exposure induces upregulation of specific microRNAs in zebrafish embryos
Author: Soares, Ana Raquel
Pereira, Patrícia M.
Ferreira, Violeta
Reverendo, Marisa
Simões, João
Bezerra, Ana Rita
Moura, Gabriela R.
Santos, Manuel A. S.
Keywords: Ethanol toxicity
MicroRNAs
Zebrafish
Apoptosis
Issue Date: May-2012
Publisher: Oxford University Press
Abstract: Prenatal exposure to ethanol leads to a myriad of developmental disorders known as fetal alcohol spectrum disorder, often characterized by growth and mental retardation, central nervous system damage, and specific craniofacial dysmorphic features. The mechanisms of ethanol toxicity are not fully understood, but exposure during development affects the expression of several genes involved in cell cycle control, apoptosis, and transcriptional regulation. MicroRNAs (miRNAs) are implicated in some of these processes, however, it is not yet clear if they are involved in ethanol-induced toxicity. In order to clarify this question, we have exposed zebrafish embryos to ethanol and evaluated whether a miRNA deregulation signature could be obtained. Zebrafish embryos were exposed to 1 and 1.5% of ethanol from 4 h postfertilization (hpf) to 24 hpf. The miRNA expression profiles obtained reveal significant miRNA deregulation and show that both ethanol concentrations upregulate miR-153a, miR-725, miR-30d, let-7k, miR-100, miR-738, and miR-732. Putative gene targets of deregulated miRNAs are involved in cell cycle control, apoptosis, and transcription, which are the main processes affected by ethanol toxicity. The conservation of affected mechanisms among vertebrates leads us to postulate that similar miRNA deregulation occurs in humans, highlighting a relevant role of miRNAs in ethanol toxicology.
Peer review: yes
URI: http://hdl.handle.net/10773/26712
DOI: 10.1093/toxsci/kfs068
ISSN: 1096-6080
Appears in Collections:CESAM - Artigos
DBio - Artigos

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