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dc.contributor.authorSoares, Ana R.pt_PT
dc.contributor.authorReverendo, Marisapt_PT
dc.contributor.authorPereira, Patrícia M.pt_PT
dc.contributor.authorNivelles, Olivierpt_PT
dc.contributor.authorPendeville, Hélènept_PT
dc.contributor.authorBezerra, Ana Ritapt_PT
dc.contributor.authorMoura, Gabriela R.pt_PT
dc.contributor.authorStruman, Ingridpt_PT
dc.contributor.authorSantos, Manuel A. S.pt_PT
dc.description.abstractBackground - MicroRNAs (miRNAs) are a class of small RNAs that are implicated in the control of eukaryotic gene expression by binding to the 3′UTR of target mRNAs. Several algorithms have been developed for miRNA target prediction however, experimental validation is still essential for the correct identification of miRNA targets. We have recently predicted that Neuropilin2a (Nrp2a), a vascular endothelial growth factor receptor which is essential for normal developmental angiogenesis in zebrafish, is a dre-miR-2188 target. Methodology - Here we show that dre-miR-2188 targets the 3′-untranslated region (3′UTR) of Nrp2a mRNA and is implicated in proper intersegmental vessel development in vivo. Over expression of miR-2188 in zebrafish embryos down regulates Nrp2a expression and results in intersegmental vessel disruption, while its silencing increases Nrp2a expression and intersegmental vessel sprouting. An in vivo GFP sensor assay based on a fusion between the GFP coding region and the Nrp2a 3′UTR confirms that miR-2188 binds to the 3′UTR of Nrp2a and inhibits protein translation. Conclusions - We demonstrate that miR-2188 targets Nrp2a and affects intersegmental vessel development in zebrafish embryos.pt_PT
dc.publisherPublic Library of Sciencept_PT
dc.titleDre-miR-2188 targets Nrp2a and mediates proper intersegmental vessel development in zebrafish embryospt_PT
degois.publication.firstPage1 - e39417pt_PT
degois.publication.lastPage11 - e39417pt_PT
degois.publication.titlePloS onept_PT
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