Utilize este identificador para referenciar este registo: http://hdl.handle.net/10773/26615
Título: Contribution of reactive oxygen species to UV-B-induced damage in bacteria
Autor: Santos, Ana L.
Gomes, Newton C. M.
Henriques, Isabel
Almeida, Adelaide
Correia, António
Cunha, Ângela
Palavras-chave: Bacteria
UV radiation
Reactive oxygen species
Oxidative damage
Data: 2012
Editora: Elsevier
Resumo: The present work aimed to identify the reactive oxygen species (ROS) produced during UV-B exposure and their biochemical targets, in a set of bacterial isolates displaying different UV susceptibilities. For that, specific exogenous ROS scavengers (catalase/CAT, superoxide dismutase/SOD, sodium azide and mannitol) were used. Biological effects were assessed from total bacterial number, colony counts and heterotrophic activity (glucose uptake and respiration). DNA strand breakage, ROS generation, oxidative damage to proteins and lipids were used as markers of oxidative stress. Sodium azide conferred a statistically significant protection in terms of lipid oxidation and cell survival, suggesting that singlet oxygen might play an important role in UV-B induced cell inactivation. Mannitol exerted a significant protection against DNA strand breakage and protein carbonylation, assigning hydroxyl radicals to DNA and protein damage. The addition of exogenous CAT and SOD significantly protected the capacity for glucose uptake and respiration, suggesting that superoxide and H(2)O(2) are involved in the impairment of activity during UV-B exposure. The observation that amendment with ROS scavengers can sometimes also exert a pro-oxidant effect suggests that the intracellular oxidant status of the cell ultimately determines the efficiency of antioxidant defenses.
Peer review: yes
URI: http://hdl.handle.net/10773/26615
DOI: 10.1016/j.jphotobiol.2012.08.016
ISSN: 1011-1344
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