Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorCleary, Daniel F. R.pt_PT
dc.contributor.authorSmalla, Korneliapt_PT
dc.contributor.authorMendonça-Hagler, Leda C. S.pt_PT
dc.contributor.authorGomes, Newton C. M.pt_PT
dc.description.abstractHere, we use DGGE fingerprinting and barcoded pyrosequencing data, at six cut-off levels (85-100%), of all bacteria, Alphaproteobacteria and Betaproteobacteria to assess composition in the rhizosphere of nursery plants and nursery-raised transplants, native plants and bulk sediment in a mangrove habitat. When comparing compositional data based on DGGE fingerprinting and barcoded pyrosequencing at different cut-off levels, all revealed highly significant differences in composition among microhabitats. Procrustes superimposition revealed that ordination results using cut-off levels from 85-100% and DGGE fingerprint data were highly congruent with the standard 97% cut-off level. The various approaches revealed a primary gradient in composition from nursery to mangrove samples. The affinity between the nursery and transplants was greatest when using Betaproteobacteria followed by Alphaproteobacteria data. There was a distinct secondary gradient in composition from transplants to bulk sediment with native plants intermediate, which was most prevalent using all bacteria at intermediate cut-off levels (92-97%). Our results show that PCR-DGGE provides a robust and cost effective exploratory approach and is effective in distinguishing among a priori defined groups.pt_PT
dc.description.sponsorshipThis study was funded by the Deutsche Forschungsgemeinschaft SM59/4-1 and 4-2 (, FAPERJ-Brazil (, Centre for Environmental and Marine Studies (CESAM, Portugal) (, Foundation for Science and Technology (FCT, Portugal) PTDC/AAC-CLI/107916/2008 ( and the European Regional Development Fund (ERDF) through COMPETE- (FCOMP-01-0124-FEDER-008657). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.pt_PT
dc.publisherPublic Library of Sciencept_PT
dc.titleAssessment of variation in bacterial composition among microhabitats in a mangrove environment using DGGE fingerprints and barcoded pyrosequencingpt_PT
degois.publication.firstPage1- e29380pt_PT
degois.publication.lastPage8- e29380pt_PT
degois.publication.titlePloS onept_PT
Appears in Collections:CESAM - Artigos
DBio - Artigos

Files in This Item:
File Description SizeFormat 
Cleary et al. - 2012 - Assessment of Variation in Bacterial Composition a.pdf971.6 kBAdobe PDFView/Open

Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.