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|Title:||Photodynamic inactivation of Penicillium chrysogenum conidia by cationic porphyrins|
|Author:||Gomes, Maria C.|
Woranovicz-Barreira, Sandra M.
Faustino, Maria A. F.
Neves, Maria G. P. M. S.
Tomé, Augusto C.
Gomes, Newton C. M.
Cavaleiro, José A. S.
Tomé, João P. C.
|Publisher:||Royal Society of Chemistry|
|Abstract:||This work reports the photophysical and biological evaluation of ﬁve cationic porphyrins as photosensitizers (PS) for the photodynamic inactivation (PDI) of Penicillium chrysogenum conidia. Two different cationic porphyrin groups were synthesized from 5,10,15,20-tetrakis(4-pyridyl)porphyrin and 5,10,15,20-tetrakis(pentaﬂuorophenyl)porphyrin. The photostability and singlet oxygen generation studies showed that these molecules are photostable and efﬁcient singlet oxygen generators. PDI experiments of P. chrysogenum conidia conducted with 50 mmol L-1 of photosensitiser under white light at a ﬂuence rate of 200 mW cm-2 over 20 min showed that the most effective PS caused a 4.1 log reduction in the concentration of viable conidia. The present results show that porphyrins 1a and 1b are more efﬁcient PSs than porphyrin 2a while porphyrins 1c and 2b show no inactivation of P. chrysogenum. It is also clear that the effectiveness of the molecule as PS for antifungal PDI is strongly related with the porphyrin substituent groups, and consequently their solubility in physiological media. The average amount of PS adsorbed per viable conidium was a determining factor in the photoinactivation efﬁciency and varied between the different studied PSs. Cationic PSs 1a and 1b might be promising anti-fungal PDI agents with potential applications in phytosanitation, bioﬁlm control, bioremediation, and wastewater treatment.|
|Appears in Collections:||CESAM - Artigos|
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