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dc.contributor.authorRodrigues, Sara R.pt_PT
dc.contributor.authorCaldeira, Carinapt_PT
dc.contributor.authorCastro, Bruno B.pt_PT
dc.contributor.authorGonçalves, Fernandopt_PT
dc.contributor.authorNunes, Brunopt_PT
dc.contributor.authorAntunes, Sara C.pt_PT
dc.description.abstractInhibition of cholinesterases (ChEs) has been widely used as an environmental biomarker of exposure to organophosphates (OP) and carbamate (CB) pesticides. More recently, this biomarker has been suggested as a putative biomarker for exposure to detergents. The use of cholinesterase inhibition as effect criterion in Ecotoxicology requires the previous characterization of the specific enzymatic forms that may be present in different tissues or organs. Different ChEs isoforms may be present in the same tissue and may exhibit distinct sensitivities towards environmental contaminants. This work intended to characterize the soluble ChEs present in pumpkinseed sunfish (Lepomis gibbosus) total head and dorsal muscle homogenates, through the use of different substrates and selective inhibitors of cholinesterasic activity. Also, the in vitro effects of sodium dodecylsulphate (SDS – anionic detergent) and chlorfenvinphos (organophosphate pesticide) on the enzymatic activity of the mentioned species were investigated. In general terms, the predominant cholinesterasic form present in both tissues was acetylcholinesterase. Chlorfenvinphos was responsible for inhibitory effects on AChE activity, while SDS did not cause any significant effect. These results suggest that in environmental monitoring programs, L. gibbosus head and dorsal muscle AChE can be an adequate diagnostic tool for exposure to OP pesticides; this conclusion however is not applicable to detergent residues. We also discuss the usefulness of L. gibbosus as an alternative model system and valuable option for freshwater ecotoxicological monitoring programs.pt_PT
dc.description.sponsorshipThe present work was funded by project “BiOtoMetal” (PTDC/AMB/70431/2006) from the Portuguese Foundation for Science and Technology (FCT, Fundação para a Ciência e a Tecnologia). FCT also financed Sara C. Antunes with a Post-doctoral fellowship (SFRH/BPD/40052/2007). Bruno B. Castro is hired under the programme Ciência 2008 (FCT, Portugal), co-funded by the Human Potential Operational Programme (National Strategic Reference Framework 2007–2013) and European Social Fund (EU).pt_PT
dc.subjectLepomis gibbosuspt_PT
dc.subjectCholinesterases characterizationpt_PT
dc.subjectEnvironmental monitoringpt_PT
dc.subjectEnzyme inhibitionpt_PT
dc.subjectOrganophosphate pesticidept_PT
dc.subjectAlternative model fish systempt_PT
dc.subjectAcetylcholinesterase (AChE) activitypt_PT
dc.subjectIn vivo assayspt_PT
dc.subjectIn vitro assayspt_PT
dc.titleCholinesterase (ChE) inhibition in pumpkinseed (Lepomis gibbosus) as environmental biomarker: ChE characterization and potential neurotoxic effects of xenobioticspt_PT
degois.publication.titlePesticide Biochemistry and Physiologypt_PT
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