Please use this identifier to cite or link to this item:
Title: Biochemical characterization of Sfh-I, a subclass B2 metallo-β-Lactamase from a Serratia fonticola UTAD54
Author: Fonseca, Fátima
Arthur, Christopher J.
Bromley, Elizabeth H. C.
Samyn, Bart
Moerman, Pablo
Saavedra, Maria José
Correia, António
Spencer, James
Issue Date: 2011
Publisher: American Society for Microbiology
Abstract: The subclass B2 metallo-β-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and overexpressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBLs, Sfh-I also shows limited hydrolytic activity against some additional substrates and is not inhibited by a second equivalent of zinc. These data confirm Sfh-I to be a subclass B2 metallo-β-lactamase with some distinctive properties.
Peer review: yes
DOI: 10.1128/AAC.00429-11
ISSN: 0066-4804
Appears in Collections:CESAM - Artigos
DBio - Artigos

Files in This Item:
File Description SizeFormat 
Fonseca et al. - 2011 - Biochemical Characterization of Sfh-I, a Subclass .pdf675.17 kBAdobe PDFView/Open

Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.