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Title: Acclimatization of secondary somatic embryos derived plants of Eucalyptus globulus Labill.: an ultrastructural approach
Author: Pinto, Glória
Silva, Sónia
Loureiro, João
Costa, Armando
Dias, Maria Celeste
Araújo, Clara
Neves, Lucinda
Santos, Conceição
Keywords: Histological differentiation
Plant acclimatization
Ploidy stability
Secondary somatic embryos
Ultrastructural studies
Issue Date: 2011
Publisher: Springer Verlag
Abstract: This paper reports the complete process from secondary emblings (SE-derived plants) regeneration to acclimatization of Eucalyptus globulus and describes histocytological changes that occur in leaves from in vitro to ex vitro acclimatization for a 3-month period. After elongation, plantsweretransferredtopotswithsterilizedpeat:perliteand acclimatized in a phytotron, with progressive reduction of RH and increaseoflight intensity.Histocytologicalanalyses were performed in fixed material using lightmicroscopy and ultrastructural changes followed by electron microscopy (SEM and TEM). The protocol used allowed the successful acclimatization of the emblings. Plants looked morphologically normal and FCM screening revealed no ploidy or DNA content abnormalities. Histocytological analyses showed significant changes along time, mostly in stomata shape and aperture, starch reserves, chloroplast morphology and mesophyll differentiation. This is the first report concerning emblings acclimatization to ex vitro conditions in Eucalyptus. It was clearly demonstrated that during acclimatization emblings suffered profound changes in leaf morphology in order to successfully adapt to ex vitro conditions.
Peer review: yes
DOI: 10.1007/s00468-010-0513-y
ISSN: 0931-1890
Appears in Collections:CESAM - Artigos
DBio - Artigos

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