Please use this identifier to cite or link to this item:
|Title:||Mechanisms of response to silver nanoparticles on Enchytraeus albidus (Oligochaeta): survival, reproduction and gene expression profile|
|Author:||Gomes, Susana I. L.|
Soares, Amadeu M. V. M.
Scott-Fordsmand, Janeck J.
Amorim, Mónica J. B.
|Abstract:||Silver has antimicrobial properties and silver nanoparticles (Ag-NPs) have been some of the most widely used NPs. Information regarding their effects is still insufficient, in particular for soil dwelling organisms. The standard soil Oligochaete Enchytraeus albidus was used to study the effects of Ag in soils, using differential gene expression (microarray) and population (survival, reproduction) response to Ag-NPs (PVP coated) and AgNO3. Results showed higher toxicity of AgNO3(EC50<50mg/kg) compared to toxicity of Ag-NPs (EC50=225mg/kg). Based on the biological and material identity, the difference in toxicity between Ag-NPs and AgNO3could possibly be explained by a release of Ag+ions from the particles or by a slower uptake of Ag-NPs. The indications were that the responses to Ag-NPs reflect an effect of Ag ions and Ag-NPs given the extent of similar/dissimilar genes activated. The particles characterization supports this deduction as there were limited free ions measured in soil extracts, maybe related to little oxidation and/or complexation in the soil matrix. The possibility that gene differences were due to different levels of biological impact (i.e. physiological responses) should not be excluded. Testing of Ag-NPs seem to require longer exposure period to be comparable in terms of effect/risk assessment with other chemicals.|
|Appears in Collections:||CESAM - Artigos|
DBio - Artigos
Files in This Item:
|Gomes et al. - 2013 - Mechanisms of response to silver nanoparticles on .pdf||1.52 MB||Adobe PDF|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.