Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/23265
Title: | Comparative assessment of intrinsic mechanical stimuli on knee cartilage and compressed agarose constructs |
Author: | Completo, António Bandeiras, Cátia Fonseca, Fernando |
Keywords: | Finite element models Tibiofemoral contact Pore pressure Maximal principal strain Fluid velocity Ttissue-engineerin |
Issue Date: | 2017 |
Publisher: | Elsevier |
Abstract: | A well established cue for improving the properties of tissue-engineered cartilage is mechanical stimulation. However, the explicit ranges of mechanical stimuli that correspond to favourable metabolic outcomes are elusive. Usually, these outcomes have only been associated with the applied strain and frequency, an oversimplification that can hide the fundamental relationship between the intrinsic mechanical stimuli and the metabolic outcomes. This highlights two important key issues: the firstly is related to the evaluation of the intrinsic mechanical stimuli of native cartilage; the second, assuming that the intrinsic mechanical stimuli will be important, deals with the ability to replicate them on the tissue-engineered constructs. This study quantifies and compares the volume of cartilage and agarose subjected to a given magnitude range of each intrinsic mechanical stimulus, through a numerical simulation of a patient-specific knee model coupled with experimental data of contact during the stance phase of gait, and agarose constructs under direct-dynamic compression. The results suggest that direct compression loading needs to be parameterized with time-dependence during the initial culture period in order to better reproduce each one of the intrinsic mechanical stimuli developed in the patient-specific cartilage. A loading regime which combines time periods of low compressive strain (5%) and frequency (0.5Hz), in order to approach the maximal principal strain and fluid velocity stimulus of the patient-specific cartilage, with time periods of high compressive strain (20%) and frequency (3Hz), in order to approach the pore pressure values, may be advantageous relatively to a single loading regime throughout the full culture period |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/23265 |
DOI: | 10.1016/j.medengphy.2017.02.013 |
ISSN: | 1350-4533 |
Appears in Collections: | DEM - Artigos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Author Manuscript_Comparative assessment of intrinsic mechanical stimuli on knee cartilage and compressed agarose constructs.pdf | 502.94 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.