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Title: Evaluation of the anthropogenic input of caffeine in surface waters of the north and center of portugal by ELISA
Author: Silva, Carla Patrícia
Lima, Diana L. D.
Schneider, Rudolf J.
Otero, Marta
Esteves, Valdemar I.
Keywords: Anthropogenic markers
Surface water
Issue Date: 2014
Publisher: Elsevier
Abstract: This study comprises the development of an enzyme-linked immunosorbent assay (ELISA) for the quantification of caffeine in complex aqueous matrices without any sample clean-up procedure. Salinity and dissolved organic matter were selected as potential interfering agents. The addition of a sample buffer containing bovine serum albumin (BSA) prior to the sample was found to decrease the influence of those interfering agents. The working range of the developed method was 0.1-100??gL-1. Quantification of caffeine was possible in 43 out of 51 real aqueous samples, at values between <LOD and 15??gL-1. Results correlate well with those obtained by LC-MS/MS. To the best of our knowledge this is the first study dealing with the quantification of caffeine in Portugal's surface waters.
Peer review: yes
DOI: 10.1016/j.scitotenv.2014.01.120
ISSN: 0048-9697
Appears in Collections:CESAM - Artigos
DQ - Artigos

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