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|Title:||Evaluation of the anthropogenic input of caffeine in surface waters of the north and center of portugal by ELISA|
|Author:||Silva, Carla Patrícia|
Lima, Diana L. D.
Schneider, Rudolf J.
Esteves, Valdemar I.
|Abstract:||This study comprises the development of an enzyme-linked immunosorbent assay (ELISA) for the quantification of caffeine in complex aqueous matrices without any sample clean-up procedure. Salinity and dissolved organic matter were selected as potential interfering agents. The addition of a sample buffer containing bovine serum albumin (BSA) prior to the sample was found to decrease the influence of those interfering agents. The working range of the developed method was 0.1-100??gL-1. Quantification of caffeine was possible in 43 out of 51 real aqueous samples, at values between <LOD and 15??gL-1. Results correlate well with those obtained by LC-MS/MS. To the best of our knowledge this is the first study dealing with the quantification of caffeine in Portugal's surface waters.|
|Appears in Collections:||CESAM - Artigos|
DQ - Artigos
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|Silva et al. - 2014 - Evaluation of the anthropogenic input of caffeine .pdf||346.22 kB||Adobe PDF|
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