Please use this identifier to cite or link to this item:
Title: Cadmium-induced genotoxicity in human osteoblast-like cells
Author: Oliveira, Helena
Monteiro, Cristina
Pinho, Francisco
Pinho, Sónia
Ferreira de Oliveira, José Miguel P.
Santos, Conceição
Keywords: Cadmium
Cell cycle
DNA damage
Issue Date: 2014
Publisher: Elsevier
Abstract: Cadmium (Cd) is a widespread heavy metal used in numerous industrial processes. Cd exerts toxicological effects mostly in kidney and liver. Bone is also an important target of Cd, however, the cellular mechanisms of Cd toxicological effects in the bone cells are still poorly understood. Therefore, the present work aimed to investigate the putative cytotoxic and genotoxic effects of Cd to human bone cells. For that, the osteoblast-like MG-63 cells were exposed to 20 and 50. ??M Cd for 24 and 48. h. Results showed a dose-dependent increase in Cd accumulation in cells and a decrease in cell viability, especially after 48. h. Cell cycle analysis showed a delay at S phase concomitant with a decrease in cells at G0/G1 phase. After 24. h, Cd treatment downregulated the expression of CHEK1, CHEK2 and CDK2 genes and upregulated the expression of CCNE1 gene. After 48. h, the expression of ATM and CCNB1 genes were downregulated. Also, a 3.3 fold increase on the expression of gene CCNE1 was detected. Both Cd doses induced DNA fragmentation at 48. h, while an increase in micronuclei (MN) and nucleoplasmic bridges (NPBs) together with an increase in the percentage of apoptotic/necrotic cells was detected for both time periods. Overall, our results demonstrate the cytotoxicity and genotoxicity of Cd in human bone cells. Also, the cytokinesis-block micronucleus (CBMN) assay parameters (MN, NPBs and the percentage of cells under apoptosis or necrosis) together with the cell cycle appear as the most sensitive to Cd cyto- and genotoxicity, being early affected even with the lowest Cd dose. Therefore, these cyto-/genotoxic techniques may be selected for early detection of Cd-induced toxicity.
Peer review: yes
DOI: 10.1016/j.mrgentox.2014.10.002
ISSN: 1383-5718
Appears in Collections:CESAM - Artigos
DBio - Artigos

Files in This Item:
File Description SizeFormat 
Oliveira et al. - 2014 - Cadmium-induced genotoxicity in human osteoblast-l.pdf2.08 MBAdobe PDFrestrictedAccess

Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.