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|Title:||Are DNA-damaging effects induced by herbicide formulations (Roundup® and Garlon®) in fish transient and reversible upon cessation of exposure?|
Santos, M. A.
|Keywords:||DNA damage recovery|
|Abstract:||Owing to the seasonality of crop cultivation and subsequent periodic/seasonal application of herbicides, their input to the aquatic systems is typically intermittent. Consequently, exposure of fish to this type of contaminants can be short and followed by a period of permanence in non-contaminated areas. Thus, the assessment of genotoxic endpoints in fish after removal of the contamination source appears as a crucial step to improve the knowledge on the dynamics of herbicide genotoxicity, as well as to determine the actual magnitude of risk posed by these agrochemicals. Therefore, the present study intended to shed light on the ability of fish to recover from the DNA damage induced by short-term exposures to the herbicide formulations Roundup?? (glyphosate-based) and Garlon?? (triclopyr-based) upon the exposure cessation. European eel (Anguilla anguilla) was exposed to the above commercial formulations for 3 days, and allowed to recover for 1, 7 and 14 days (post-exposure period). The comet assay was used to identify the DNA damage in blood cells during both exposure and post-exposure periods. As an attempt to clarify the DNA damaging mechanisms involved, an extra-step including the incubation of the nucleotides with DNA lesion-specific repair enzyme was added to the standard comet. The genotoxic potential of both herbicides was confirmed, concerning the exposure period. In addition, the involvement of oxidative DNA damage on the action of Roundup?? (pointed out as pyrimidine bases oxidation) was demonstrated, while for Garlon?? this damaging mechanism was less evident. Fish exposed to Garlon??, though presenting some evidence towards a tendency of recovery, did not achieve a complete restoration of DNA integrity. In what concerns to Roundup??, a recovery was evident when considering non-specific DNA damage on day 14 post-exposure. In addition, this herbicide was able to induce a late oxidative DNA damage (day 14). Blood cells of A. anguilla exposed to Roundup?? appeared to be more successful in repairing damage with a non-specific cause than that associated to base oxidation. Overall, the present findings highlighted the genetic hazard to fish associated to the addressed agrochemicals, reinforcing the hypothesis of long-lasting damage.|
|Appears in Collections:||CESAM - Artigos|
DBio - Artigos
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|Guilherme et al. - 2014 - Are DNA-damaging effects induced by herbicide form.pdf||838.51 kB||Adobe PDF|
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