Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/22512
Title: The use of cholinesterase as potential biomarker: in vitro characterization in the polychaete Capitella teleta
Author: Gomes, Isa D. L.
Lemos, Marco F. L.
Soares, Amadeu M. V. M.
Barata, Carlos
Faria, Melissa
Keywords: Acetylcholinesterase
Capitela teleta
Insecticide
Marine
Organophosphorous
Sediment
Issue Date: 2014
Publisher: Elsevier
Abstract: The ecological relevance of polychaetes coupled with their easy culture and maintenance in the laboratory, has led them to become increasingly used in marine ecotoxicological studies, raising the need to validate frequently applied monitoring tools at various biological levels. The present study was aimed to characterize the cholinesterases (ChE) activity in the polychaete Capitella teleta, using three substrates (acetylthiocholine iodide, propionylthiocholine iodide, and S-butyrylthiocholine iodide) and four known inhibitors (eserine hemisulfate, BW284c51, iso-OMPA and chlorpyrifos-oxon). Results showed that most of the measured cholinesterase activity was acetylcholinesterase (AChE). Inhibition of enzyme kinetic experiments denoted that sensitivity of C. teleta's ChE to the organophosphorous metabolite chlorpyrifos-oxon (IC50=60.72nM) was analogous to some fish species. This study highlights the relevance of ChE characterization before its use as a biomarker in ecotoxicology and biomonitoring studies.
Peer review: yes
URI: http://hdl.handle.net/10773/22512
DOI: 10.1016/j.marpolbul.2014.05.053
ISSN: 0025-326X
Appears in Collections:CESAM - Artigos
DBio - Artigos

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