Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/20786
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dc.contributor.authorVillaverde, Juan Josept
dc.contributor.authorvan der Vlist, Vincentpt
dc.contributor.authorSantos, Sonia A. O.pt
dc.contributor.authorHaarmann, Thomaspt
dc.contributor.authorLangfelder, Kimpt
dc.contributor.authorPirttimaa, Minnipt
dc.contributor.authorNyyssola, Anttipt
dc.contributor.authorJylha, Sirpapt
dc.contributor.authorTamminen, Tarjapt
dc.contributor.authorKruus, Kristiinapt
dc.contributor.authorde Graaff, Leopt
dc.contributor.authorNeto, Carlos Pascoalpt
dc.contributor.authorSimoes, Mario M. Q.pt
dc.contributor.authorDomingues, M. R. M.pt
dc.contributor.authorSilvestre, Armando J. D.pt
dc.contributor.authorEidner, Jasminpt
dc.contributor.authorBuchert, Johannapt
dc.date.accessioned2017-12-07T19:59:22Z-
dc.date.issued2013pt
dc.identifier.issn1385-8947pt
dc.identifier.urihttp://hdl.handle.net/10773/20786-
dc.description.abstractLinoleic acid was converted into hydroperoxides by a Gaeumannomyces graminis tritici lipoxygenase produced recombinantly in Trichoderma reesei. Hydroperoxide production was optimized using a face-centred experimental design in order to study the effects of pH, temperature and time on the conversion of linoleic acid into four regioisomeric hydroperoxyoctadecadienoic acids (HPODE): 13-(Z,E)-, 9-(E,Z)-, 13-(E,E)-, 9-(E,E)-HPODE. Fitting equations described satisfactorily the system behavior and showed that reaction time was the most influencing independent variable. A set of independent variables (pH = 6.7, temperature = 23.9 degrees C and time = 18 h) allowed to obtain high yields of hydroperoxides (88.0%) with a good selectivity for the 13-(Z,E)-HPODE isomer (47.4%) when the initial substrate concentration was 10 g/L. The production was further investigated using industrially relevant linoleic acid concentrations (100-300 g/L) leading to HPODE yields of similar to 40% and the volumetric productivity 3.6 g/(L h), and a selectivity for 13-(Z,E)-HPODE of around 74%. (c) 2012 Elsevier B.V. All rights reserved.pt
dc.language.isoengpt
dc.publisherELSEVIER SCIENCE SApt
dc.relationinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/109581/PTpt
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F42021%2F2007/PTpt
dc.relationinfo:eu-repo/grantAgreement/FCT/COMPETE/132936/PTpt
dc.rightsrestrictedAccesspor
dc.subjectUNSATURATED FATTY-ACIDSpt
dc.subjectSOYBEAN LIPOXYGENASEpt
dc.subjectIMMOBILIZED LIPOXYGENASEpt
dc.subjectMANGANESE LIPOXYGENASEpt
dc.subjectMASS-SPECTROMETRYpt
dc.subjectOXIDATIONpt
dc.subjectIDENTIFICATIONpt
dc.subjectSYSTEMpt
dc.subjectSTATEpt
dc.subjectOILpt
dc.titleHydroperoxide production from linoleic acid by heterologous Gaeumannomyces graminis tritici lipoxygenase: Optimization and scale-uppt
dc.typearticlept
dc.peerreviewedyespt
ua.distributioninternationalpt
degois.publication.firstPage82pt
degois.publication.lastPage90pt
degois.publication.titleCHEMICAL ENGINEERING JOURNALpt
degois.publication.volume217pt
dc.date.embargo10000-01-01-
dc.relation.publisherversion10.1016/j.cej.2012.11.090pt
dc.identifier.doi10.1016/j.cej.2012.11.090pt
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