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|Title:||Hydroperoxide production from linoleic acid by heterologous Gaeumannomyces graminis tritici lipoxygenase: Optimization and scale-up|
|Author:||Villaverde, Juan Jose|
van der Vlist, Vincent
Santos, Sonia A. O.
de Graaff, Leo
Neto, Carlos Pascoal
Simoes, Mario M. Q.
Domingues, M. R. M.
Silvestre, Armando J. D.
|Publisher:||ELSEVIER SCIENCE SA|
|Abstract:||Linoleic acid was converted into hydroperoxides by a Gaeumannomyces graminis tritici lipoxygenase produced recombinantly in Trichoderma reesei. Hydroperoxide production was optimized using a face-centred experimental design in order to study the effects of pH, temperature and time on the conversion of linoleic acid into four regioisomeric hydroperoxyoctadecadienoic acids (HPODE): 13-(Z,E)-, 9-(E,Z)-, 13-(E,E)-, 9-(E,E)-HPODE. Fitting equations described satisfactorily the system behavior and showed that reaction time was the most influencing independent variable. A set of independent variables (pH = 6.7, temperature = 23.9 degrees C and time = 18 h) allowed to obtain high yields of hydroperoxides (88.0%) with a good selectivity for the 13-(Z,E)-HPODE isomer (47.4%) when the initial substrate concentration was 10 g/L. The production was further investigated using industrially relevant linoleic acid concentrations (100-300 g/L) leading to HPODE yields of similar to 40% and the volumetric productivity 3.6 g/(L h), and a selectivity for 13-(Z,E)-HPODE of around 74%. (c) 2012 Elsevier B.V. All rights reserved.|
|Appears in Collections:||CICECO - Artigos|
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|Hydroperoxide production from linoleic acid by heterologous Gaeumannomyces graminis tritici lipoxygenase Optimization and scale-up_10.1016j.cej.2012.11.090.pdf||560.18 kB||Adobe PDF|
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