Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/20695
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dc.contributor.authorMartins, Margaridapt
dc.contributor.authorVieira, Flavia A.pt
dc.contributor.authorCorreia, Isabelpt
dc.contributor.authorFerreira, Rute A. S.pt
dc.contributor.authorAbreu, Helenapt
dc.contributor.authorCoutinho, Joao A. P.pt
dc.contributor.authorVentura, Sonia P. M.pt
dc.date.accessioned2017-12-07T19:56:10Z-
dc.date.issued2016pt
dc.identifier.issn1463-9262pt
dc.identifier.urihttp://hdl.handle.net/10773/20695-
dc.description.abstractBioactive compounds extracted from natural renewable sources have attracted increased interest from both industry and academia. Several biocompounds are present in red macroalgae, among which R-phycoerythrin (R-PE), which is a phycobiliprotein with a wide range of applications. The major drawback associated with it is the absence of an efficient, low cost and green extraction and purification methodology capable of recovering phycobiliproteins (and, in particular, R-phycoerythrin) from the biomass, while maintaining their structure and activity. The search for novel and higher performance extraction processes is thus of extreme relevance. In this work, aqueous solutions of ionic liquids were screened for the extraction of phycobiliproteins from Gracilaria sp. The most promising solvents were identified and operational conditions such as extraction time, solid-liquid ratio, solvent concentration and pH were optimized aiming to develop a new and more efficient approach to extract phycobiliproteins. The efficiency of the proposed process is demonstrated with aqueous solutions of cholinium chloride, since the extraction of phycobiliproteins was increased to 46.5% when compared with the conventional methodology, while the protein secondary structure and the chromophore conformation integrity are maintained.pt
dc.language.isoengpt
dc.publisherROYAL SOC CHEMISTRYpt
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147332/PTpt
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F79263%2F2011/PTpt
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147216/PTpt
dc.rightsrestrictedAccesspor
dc.subjectEXPANDED-BED-ADSORPTIONpt
dc.subjectR-PHYCOERYTHRINpt
dc.subjectPORPHYRIDIUM-CRUENTUMpt
dc.subjectPALMARIA-PALMATApt
dc.subjectB-PHYCOERYTHRINpt
dc.subjectPURIFICATIONpt
dc.subjectEXTRACTIONpt
dc.subjectALGApt
dc.subjectRHODOPHYTApt
dc.subjectBANGIALESpt
dc.titleRecovery of phycobiliproteins from the red macroalga Gracilaria sp using ionic liquid aqueous solutionspt
dc.typearticlept
dc.peerreviewedyespt
ua.distributioninternationalpt
degois.publication.firstPage4287pt
degois.publication.issue15pt
degois.publication.lastPage4296pt
degois.publication.titleGREEN CHEMISTRYpt
degois.publication.volume18pt
dc.date.embargo10000-01-01-
dc.relation.publisherversion10.1039/c6gc01059hpt
dc.identifier.doi10.1039/c6gc01059hpt
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