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http://hdl.handle.net/10773/20577
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pinto, Joana | pt |
dc.contributor.author | Domingues, M. Rosario M. | pt |
dc.contributor.author | Galhano, Eulalia | pt |
dc.contributor.author | Pita, Cristina | pt |
dc.contributor.author | Almeida, Maria do Ceu | pt |
dc.contributor.author | Carreira, Isabel M. | pt |
dc.contributor.author | Gil, Ana M. | pt |
dc.date.accessioned | 2017-12-07T19:52:04Z | - |
dc.date.issued | 2014 | pt |
dc.identifier.issn | 0003-2654 | pt |
dc.identifier.uri | http://hdl.handle.net/10773/20577 | - |
dc.description.abstract | This work contributes to fill in some existing gaps in the knowledge of human plasma degradability during handling and storage, a paramount issue in Nuclear Magnetic Resonance (NMR) metabolomics. Regarding the comparison between heparin and EDTA anti-coagulant collection tubes, the former showed no interference of the polysaccharide, while conserving full spectral information. In relation to time/temperature conditions, room temperature was seen to have a large impact on lipoproteins and choline compounds from 2.5 hours. In addition, short-term storage at -20 degrees C was found suitable up to 7 days but, for periods up to 1 month, -80 degrees C was recommended. Furthermore, in the case of reusing plasma samples, no more than 3 consecutive freeze-thaw cycles were found advisable. Finally, the impact of long-term -80 degrees C storage (up to 2.5 years) was found almost negligible, as evaluated on a partially matched non-fasting cohort (n = 49), after having investigated the possible confounding nature of the particular non-fasting conditions employed. | pt |
dc.language.iso | eng | pt |
dc.publisher | ROYAL SOC CHEMISTRY | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/132936/PT | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/132997/PT | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F73343%2F2010/PT | pt |
dc.rights | restrictedAccess | por |
dc.subject | HUMAN BLOOD | pt |
dc.subject | H-1-NMR | pt |
dc.subject | SERUM | pt |
dc.subject | ANTICOAGULANT | pt |
dc.subject | SPECTROSCOPY | pt |
dc.subject | VALIDATION | pt |
dc.subject | COLLECTION | pt |
dc.subject | SPECTRA | pt |
dc.subject | URINE | pt |
dc.title | Human plasma stability during handling and storage: impact on NMR metabolomics | pt |
dc.type | article | pt |
dc.peerreviewed | yes | pt |
ua.distribution | international | pt |
degois.publication.firstPage | 1168 | pt |
degois.publication.issue | 5 | pt |
degois.publication.lastPage | 1177 | pt |
degois.publication.title | ANALYST | pt |
degois.publication.volume | 139 | pt |
dc.date.embargo | 10000-01-01 | - |
dc.relation.publisherversion | 10.1039/c3an02188b | pt |
dc.identifier.doi | 10.1039/c3an02188b | pt |
Appears in Collections: | CICECO - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Human plasma stability during handling and storage impact on NMR metabolomics_10.1039c3an02188b.pdf | 804.96 kB | Adobe PDF |
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