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http://hdl.handle.net/10773/20009
Title: | Effects of emerging contaminants on neurotransmission and biotransformation in marine organisms - An in vitro approach |
Author: | Luis, Luis G. Barreto, Angela Trindade, Tito Soares, Amadeu M. V. M. Oliveira, Miguel |
Keywords: | MUSSEL MYTILUS-GALLOPROVINCIALIS POMATOSCHISTUS-MICROPS TELEOSTEI GLUTATHIONE S-TRANSFERASES HEAVY-METAL INHIBITION ZEBRAFISH DANIO-RERIO FRESH-WATER MUSSELS SHORT-TERM EXPOSURE GOLD NANOPARTICLES WASTE-WATER ACETYLCHOLINESTERASE ACTIVITY |
Issue Date: | 2016 |
Publisher: | PERGAMON-ELSEVIER SCIENCE LTD |
Abstract: | The effects of gold (ionic form and nanopartides - AuNPs) and pharmaceuticals (carbamazepine and fluoxetine) on enzymes involved in neurotransmission (acetylcholinesterase - AChE) and biotransformation (glutathione S-transferases - GST) were assessed by their incubation with Mytilus galloprovincialis' hemolymph and subcellular fraction of gills, respectively. AuNPs did not alter enzymatic activities unlike ionic gold that inhibited AChE and GST activities at 2.5 and 0.42 mg.L-1, respectively. Carbamazepine inhibited AChE activity at 500 mg.L-1 and fluoxetine at 1000 mg.L-1. GST was inhibited by carbamazepine at 250 mg.L-1 and by fluoxetine at 125 mg.L-1. Increased AChE activity was found in simultaneous exposures to fluoxetine and bovine serum albumin coated AuNPs (BSA-AuNPs). Concerning GST, in the simultaneous exposures, AuNPs revealed protective effects against carbamazepine (citrate and polyvinylpyrrolidone coated) and fluoxetine (citrate and BSA coated) induced inhibition. However, BSA-AuNPs increased the inhibition caused by carbamazepine. AuNPs demonstrated ability to interfere with other chemicals toxicity justifying further studies. (C) 2016 Elsevier Ltd. All rights reserved. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/20009 |
DOI: | 10.1016/j.marpolbul.2016.02.064 |
ISSN: | 0025-326X |
Publisher Version: | 10.1016/j.marpolbul.2016.02.064 |
Appears in Collections: | CICECO - Artigos |
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