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http://hdl.handle.net/10773/19327
Title: | PHA production by mixed cultures: A way to valorize wastes from pulp industry |
Author: | Queiros, Diogo Rossetti, Simona Serafim, Luisa S. |
Keywords: | POLY-BETA-HYDROXYBUTYRATE SPENT SULFITE LIQUORS POLYHYDROXYBUTYRATE PRODUCTION MICROBIAL CULTURE POLYHYDROXYALKANOATES COMMUNITY OXIDATION MOLASSES REACTOR OXYGEN |
Issue Date: | 2014 |
Publisher: | ELSEVIER SCI LTD |
Abstract: | In this work, hardwood spent sulfite liquor (HSSL), a complex feedstock originating from the pulp industry, was tested for the first time as a substrate for polyhydroxyalkanoate (PHA) production by a mixed microbial culture (MMC) under aerobic dynamic feeding (ADF) conditions. A sequencing batch reactor (SBR) fed with HSSL was operated for 67 days and the selected MMC reached a maximum PHA content of 67.6%. The MMC demonstrated a differential utilization of the carbon sources present in HSSL. Acetic acid was fully consumed, while xylose and lignosulphonates were partially consumed (30% and 14%, respectively). The selected culture was characterized by Fluorescence in Situ Hybridization (FISH). Bacteria belonging to the three main classes were identified: Alpha-(72.7 +/- 4.0%), Beta-(11.1 +/- 0.37%) and Gammaproteobacteria (10.3 +/- 0.3%). Within Alphaproteobacteria, a small amount of Paracoccus (4.2 +/- 0.51%) and Defluvicoccus related to Tetrad Forming Organisms (9.0 +/- 0.28%) were detected. (C) 2014 Elsevier Ltd. All rights reserved. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/19327 |
DOI: | 10.1016/j.biortech.2014.01.099 |
ISSN: | 0960-8524 |
Publisher Version: | 10.1016/j.biortech.2014.01.099 |
Appears in Collections: | CICECO - Artigos |
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File | Description | Size | Format | |
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PHA production by mixed cultures A way to valorize wastes from pulp industry_10.1016j.biortech.2014.01.099.pdf | 882.96 kB | Adobe PDF |
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