Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/19306
Title: | Evaluating Self-buffering Ionic Liquids for Biotechnological Applications |
Author: | Lee, Sze Ying Vicente, Filipa A. Silva, Francisca Sintra, Tania E. Taha, Mohamed Khoiroh, Ianatul Coutinho, Joao A. P. Show, Pau Loke Ventura, Sonia P. M. |
Keywords: | AQUEOUS BIPHASIC SYSTEMS FISCHERI MARINE-BACTERIA BOVINE SERUM-ALBUMIN ENHANCED EXTRACTION BIOLOGICAL-RESEARCH AQUATIC ORGANISMS GOODS BUFFERS LIPASE TOXICITY PURIFICATION |
Issue Date: | 2015 |
Publisher: | American Chemical Society |
Abstract: | A new range of Goods buffer ionic liquids (GB-ILs), displaying simultaneously the properties of ionic liquids and Goods buffers, were synthesized by a combination of Goods buffer anions (MOPSO, BES, TAPSO, and CAPSO) and tetrabutylammonium, tetrabutylphosphonium, and cholinium cations via an acidbase neutralization reaction. The activity and stability of a lipolytic enzyme from Pseudomonas cepacia in aqueous solutions of these buffers were evaluated, and the results show their advantage as media for enzymatic reactions when compared to conventional phosphate buffers. Moreover aqueous biphasic systems (ABS) composed by these GB-ILs and potassium citrate were investigated and shown to be highly effective and selective for the partitioning of the lipolytic enzyme into the GB-IL-rich phase. The results allow for the development of an efficient and biocompatible process combining the self-buffering and enzyme-stabilizing properties of the GB-ILs in the reaction step, with the advantages of GB-ILs as extraction solvents in ABS. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/19306 |
DOI: | 10.1021/acssuschemeng.5b01155 |
ISSN: | 2168-0485 |
Publisher Version: | 10.1021/acssuschemeng.5b01155 |
Appears in Collections: | CICECO - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Evaluating Self-buffering Ionic Liquids for Biotechnological Applications_10.1021acssuschemeng.5b01155.pdf | 806.75 kB | Adobe PDF |
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