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Title: High-throughput molecular profiling of a P-cadherin overexpressing breast cancer model reveals new targets for the anti-cancer bacterial protein azurin
Author: Bernardes, Nuno
Ribeiro, Ana Sofia
Abreu, Sofia
Vieira, André F.
Carreto, Laura
Santos, Manuel
Seruca, Raquel
Paredes, Joana
Fialho, Arsenio M.
Keywords: Azurin
Breast cancer
cDNA microarrays
Issue Date: 2014
Publisher: Elsevier
Abstract: Azurin is a bacterial protein from Pseudomonas aeruginosa which exerts an inhibitory activity in cancer cells. In P-cadherin-overexpressing models, a bad prognosis marker in breast cancer increasing invasion and other malignant features, azurin decreases the invasion of cancer cells. We performed a microarray analysis to compare the expression profile of azurin treated cells with different P-cadherin expression levels. Azurin up-regulated apoptosis mediated by p53 protein, endocytosis and vesicle-mediated transport. In the contrary, in invasive MCF-7/AZ.Pcad cells, azurin decreased the expression of genes associated with cell surface receptors and signal transduction, as well as biological adhesion. Further, azurin decreased adhesion of cells to proteins from the extracellular matrix (ECM) and altered protein expression of integrins α6, β4 and β1 and interfered with the ability of these cells to form mammospheres. Altogether, our results further enlighten the anti-cancer effects mediated by azurin in P-cadherin overexpression breast cancer models.
Peer review: yes
DOI: 10.1016/j.biocel.2014.01.023
ISSN: 1357-2725
Appears in Collections:CESAM - Artigos
DBio - Artigos

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