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Title: Ag nanoparticles (Ag NM300K) in the terrestrial environment: effects at population and cellular level in folsomia candida (Collembola)
Author: Mendes, Luís André
Maria, Vera L.
Scott-Fordsmand, Janeck J.
Amorim, Mónica J. B.
Keywords: antioxidant defenses
mechanisms of response
soil invertebrates
Issue Date: 2015
Publisher: MDPI
Abstract: The effects of nanomaterials have been primarily assessed based on standard ecotoxicity guidelines. However, by adapting alternative measures the information gained could be enhanced considerably, e.g., studies should focus on more mechanistic approaches. Here, the environmental risk posed by the presence of silver nanoparticles (Ag NM300K) in soil was investigated, anchoring population and cellular level effects, i.e., survival, reproduction (28 days) and oxidative stress markers (0, 2, 4, 6, 10 days). The standard species Folsomia candida was used. Measured markers included catalase (CAT), glutathione reductase (GR), glutathione S-transferase (GST), total glutathione (TG), metallothionein (MT) and lipid peroxidation (LPO). Results showed that AgNO3 was more toxic than AgNPs at the population level: reproduction EC20 and EC50 was ca. 2 and 4 times lower, respectively. At the cellular level Correspondence Analysis showed a clear separation between AgNO3 and AgNP throughout time. Results showed differences in the mechanisms, indicating a combined effect of released Ag+ (MT and GST) and of AgNPs (CAT, GR, TG, LPO). Hence, clear advantages from mechanistic approaches are shown, but also that time is of importance when measuring such responses.
Peer review: yes
DOI: 10.3390/ijerph121012530
ISSN: 1660-4601
Appears in Collections:CESAM - Artigos
DBio - Artigos

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