Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/17417
Title: | RUN and FYVE domain-containing protein 4 enhances autophagy and lysosome tethering in response to Interleukin-4 |
Author: | Terawaki, Seigo Camosseto, Voahirana Prete, Francesca Wenger, Till Papadopoulos, Alexia Rondeau, Christiane Combes, Alexis Rodrigues, Christian Rodriguez Manh, Thien-Phong Vu Fallet, Mathieu English, Luc Santamaria, Rodrigo Soares, Ana R. Weil, Tobias Hammad, Hamida Desjardins, Michel Gorvel, Jean-Pierre Santos, Manuel A. S. Gatti, Evelina Pierre, Philippe |
Issue Date: | 2015 |
Publisher: | Rockefeller University Press |
Abstract: | Autophagy is a key degradative pathway coordinated by external cues, including starvation, oxidative stress, or pathogen detection. Rare are the molecules known to contribute mechanistically to the regulation of autophagy and expressed specifically in particular environmental contexts or in distinct cell types. Here, we unravel the role of RUN and FYVE domain–containing protein 4 (RUFY4) as a positive molecular regulator of macroautophagy in primary dendritic cells (DCs). We show that exposure to interleukin-4 (IL-4) during DC differentiation enhances autophagy flux through mTORC1 regulation and RUFY4 induction, which in turn actively promote LC3 degradation, Syntaxin 17– positive autophagosome formation, and lysosome tethering. Enhanced autophagy boosts endogenous antigen presentation by MHC II and allows host control of Brucella abortus replication in IL-4–treated DCs and in RUFY4-expressing cells. RUFY4 is therefore the first molecule characterized to date that promotes autophagy and influences endosome dynamics in a subset of immune cells. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/17417 |
DOI: | 10.1083/jcb.201501059 |
ISSN: | 0021-9525 |
Appears in Collections: | CESAM - Artigos DBio - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Terawaki et al. - 2015 - RUN and FYVE domain-containing protein 4 enhances .pdf | 15.76 MB | Adobe PDF | View/Open |
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