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Title: RUN and FYVE domain-containing protein 4 enhances autophagy and lysosome tethering in response to Interleukin-4
Author: Terawaki, Seigo
Camosseto, Voahirana
Prete, Francesca
Wenger, Till
Papadopoulos, Alexia
Rondeau, Christiane
Combes, Alexis
Rodrigues, Christian Rodriguez
Manh, Thien-Phong Vu
Fallet, Mathieu
English, Luc
Santamaria, Rodrigo
Soares, Ana R.
Weil, Tobias
Hammad, Hamida
Desjardins, Michel
Gorvel, Jean-Pierre
Santos, Manuel A. S.
Gatti, Evelina
Pierre, Philippe
Issue Date: 2015
Publisher: Rockefeller University Press
Abstract: Autophagy is a key degradative pathway coordinated by external cues, including starvation, oxidative stress, or pathogen detection. Rare are the molecules known to contribute mechanistically to the regulation of autophagy and expressed specifically in particular environmental contexts or in distinct cell types. Here, we unravel the role of RUN and FYVE domain–containing protein 4 (RUFY4) as a positive molecular regulator of macroautophagy in primary dendritic cells (DCs). We show that exposure to interleukin-4 (IL-4) during DC differentiation enhances autophagy flux through mTORC1 regulation and RUFY4 induction, which in turn actively promote LC3 degradation, Syntaxin 17– positive autophagosome formation, and lysosome tethering. Enhanced autophagy boosts endogenous antigen presentation by MHC II and allows host control of Brucella abortus replication in IL-4–treated DCs and in RUFY4-expressing cells. RUFY4 is therefore the first molecule characterized to date that promotes autophagy and influences endosome dynamics in a subset of immune cells.
Peer review: yes
DOI: 10.1083/jcb.201501059
ISSN: 0021-9525
Appears in Collections:CESAM - Artigos
DBio - Artigos

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