Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/16783
Title: | Negeviruses found in multiple species of mosquitoes from southern Portugal: Isolation, genetic diversity, and replication in insect cell culture |
Author: | Carapeta, Sara do Bem, Beatriz McGuinness, James Esteves, Aida Abecasis, Ana Lopes, Ângela de Matos, António P. Piedade, João de Almeida, António P. G. Parreira, Ricardo |
Keywords: | Negevirus Mosquitoes Portugal RT-PCR Viral replication dsRNAintermediate Cytopathic effect Phylogeneticanalysis |
Issue Date: | 2015 |
Publisher: | Elsevier |
Abstract: | In this report, an RT-PCR approach based on the use of degenerate primers allowed the identification of negeviruses in four different species of mosquitoes (Ochlerotatus caspius, Culex pipiens, Cx. theileri and Cx. univittatus) collected in southern Portugal. The genomes of two of these viruses, sequenced to full completion, were shown to encode all the proteins encoded by previously described negeviruses. One of these viruses induces exuberant cytopathic effect in insect cell culture, with no obvious signs of apoptosis induction, replicating very rapidly and allowing for the detection of viral genomes in the infected culture supernatant as soon as 4 h post-infection. This virus was also shown to use a dsRNA intermediate, which was found to be fully formed and active 3 h after infection. Phylogenetic analysis of two products encoded by the viral ORF1 placed both viruses among Negev virus cluster, in the recently proposed Nelorpivirus taxon. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/16783 |
DOI: | 10.1016/j.virol.2015.04.021 |
ISSN: | 0042-6822 |
Appears in Collections: | CESAM - Artigos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Carapeta et al. - 2015 - Negeviruses found in multiple species of mosquitoe.pdf | 1.55 MB | Adobe PDF |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.