Utilize este identificador para referenciar este registo:
http://hdl.handle.net/10773/15841
Título: | Secretome analysis of Trichoderma atroviride T17 biocontrol of Guignardia citricarpa |
Autor: | Lima, Fernanda Blauth de Félix, Carina Osório, Nádia Alves, Artur Vitorino, Rui Domingues, Pedro Correia, António Ribeiro, Rute T. da Silva Esteves, Ana Cristina |
Palavras-chave: | Trichoderma atroviride Guignardia citricarpa Biological control Proteomics Pathogen antagonism |
Data: | Ago-2016 |
Editora: | Elsevier |
Resumo: | The fungal species Guignardia citricarpa is an important pathogen in citriculture. Members of the fungal genus Trichoderma are recognized as biocontrol agents but studies on the interactions between both fungi are scarce. This study aimed to identify extracellular proteins secreted by Trichoderma atroviride T17 that are related to the control of G. citricarpa. Two-dimensional gel electrophoresis (2D) was used to study the patterns of proteins secreted by T. atroviride T17 in medium containing glucose (control) and in medium containing G. citricarpa GC3 inactivated mycelium. We identified 59 of the 116 spots differentially expressed (50.86%) by LC–MS/MS. Of these, we highlight the presence of glycoside hydrolases (CAZy families 3, 43, 54, 76 and 93), chitinase, mutanase, a-1,3-glucanase, a-1,2-mannosidase, carboxylic hydrolase ester, carbohydrate-binding module family 13, glucan 1,3-b-glucosidase, a-galactosidase and Neutral protease 2. These proteins are related to mycoparasitism processes, stimuli and therefore to the biological control of pathogens. The results obtained are in agreement with reports describing an increase in the secretion of proteins related to mycoparasitism and biological control and a reduction in the secretion of proteins related to the metabolism of Trichoderma species grown in the presence of the pathogen. Moreover, these results are pioneer in understanding T. atroviride interaction with G. citricarpa. For the first time, we identified potential candidate proteins that may have a role in the antagonism mechanism of G. citricarpa by T. atroviride T17. Thus our results shed a light into the molecular mechanisms that T. atroviride use to control G. citricarpa. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/15841 |
DOI: | 10.1016/j.biocontrol.2016.04.009 |
ISSN: | 1049-9644 |
Aparece nas coleções: | CESAM - Artigos |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Artigo_T_atroviride_20_03_2016_no marked changes.docx | Pre-print | 130.67 kB | Microsoft Word XML |
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.