Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/13195
Title: Anchoring novel molecular biomarker responses to traditional responses in fish exposed to environmental contamination
Author: Nogueira, P.
Pacheco, M.
Pereira, M. L.
Mendo, S.
Rotchell, J. M.
Keywords: Pollution
Biomarker
Toxicology
Fish
Issue Date: May-2010
Publisher: Elsevier
Abstract: The responses of Dicentrarchus labrax and Liza aurata to aquatic pollution were assessed in a contaminated coastal lagoon, using both traditional and novel biomarkers combined. DNA damage, assessed by comet assay, was higher in both fish species from the contaminated sites, whereas levels of cytochrome P450 1A1 gene expression were not significantly altered. The liver histopathological analysis also revealed significant lesions in fish from contaminated sites. Alterations in ras and xpf genes were analysed and additional pollutant-responsive genes were identified. While no alterations were found in ras gene, a downregulation of xpf gene was observed in D. labrax from a contaminated site. Suppression subtractive hybridization applied to D. labrax collected at a contaminated site, revealed altered expression in genes involved in energy metabolism, immune system activity and antioxidant response. The approach and results reported herein demonstrate the utility of anchoring traditional biomarker responses alongside novel biomarker responses.
Peer review: yes
URI: http://hdl.handle.net/10773/13195
DOI: 10.1016/j.envpol.2009.11.007
ISSN: 0269-7491
Appears in Collections:CESAM - Artigos
Ria de Aveiro - Artigos

Files in This Item:
File Description SizeFormat 
anchoring novel molecular biomarker responses.pdf1.1 MBAdobe PDFrestrictedAccess


FacebookTwitterLinkedIn
Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.