Mercury accumulation patterns and biochemical endpoints in wild fish (Liza aurata): A multi-organ approach

Abstract

The integration of bioaccumulation and effect biomarkers in fish has been proposed for risk evaluation of aquatic contaminants. However, this approach is still uncommon, namely in the context of mercury contamination. Furthermore, a multi-organ evaluation allows an overall account of the organisms' condition. Having in mind the organs' role on metal toxicokinetics and toxicodynamics, gills, liver and kidney of golden grey mullet (Liza aurata) were selected and mercury accumulation, antioxidant responses and peroxidative damage were assessed. Two critical locations in terms of mercury occurrence were selected from an impacted area of the Ria de Aveiro, Portugal (L1, L2), and compared with a reference area. Although kidney was the organ with the highest mercury load, only gills and liver were able to distinguish mercury accumulation between reference (R) and contaminated stations. Each organ demonstrated different mercury burdens, whereas antioxidant responses followed similar patterns. Liver and kidney showed an adaptive capacity to the intermediate degree of contamination/accumulation (L1) depicted in a catalase activity increase. In contrast, none of the antioxidants was induced under higher contamination/accumulation (L2) in any organ, with the exception of renal GST. The lack of lipid peroxidation increase observed in the three organs denunciates the existence of an efficient antioxidant system. However, the evidences of limitations on antioxidants performance at L2 cannot be overlooked as an indication of mercury-induced toxicity. Having in mind the responses of the three organs, CAT revealed to be the most suitable parameter for identifying mercury exposure in the field. Overall, organ-specific mercury burdens were unable to distinguish the intermediate degree of contamination, while antioxidant responses revealed limitations on signalizing the worst scenario, reinforcing the need to their combined use.