Diversity in UV sensitivity and recovery potential among bacterioneuston and bacterioplankton isolates

Abstract

Aims: To assess the variability in UV-B (280–320 nm) sensitivity of selected bacterial isolates from the surface microlayer and underlying water of the Ria de Aveiro (Portugal) estuary and their ability to recover from previous UVinduced stress. Methods and Results: Bacterial suspensions were exposed to UV-B radiation (3Æ3 W m)2). Effects on culturability and activity were assessed from colony counts and 3H-leucine incorporation rates, respectively. Among the tested isolates, wide variability in UV-B-induced inhibition of culturability (37Æ4–99Æ3%) and activity (36Æ0–98Æ0%) was observed. Incubation of UV-B-irradiated suspensions under reactivating regimes (UV-A, 3Æ65 W m)2; photosynthetic reactive radiation, 40 W m)2; dark) also revealed diversity in the extent of recovery from UV-B stress. Trends of enhanced resistance of culturability (up to 15Æ0%) and enhanced recovery in activity (up to 52Æ0%) were observed in bacterioneuston isolates. Conclusions: Bacterioneuston isolates were less sensitive and recovered more rapidly from UV-B stress than bacterioplankton isolates, showing enhanced reduction in their metabolism during the irradiation period and decreased culturability during the recovery process compared to bacterioplankton. Significance and Impact of the Study: UV exposure can affect the diversity and activity of microbial communities by selecting UV-resistant strains and alter their metabolic activity towards protective strategies.