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http://hdl.handle.net/10773/11067
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DC Field | Value | Language |
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dc.contributor.author | Tacão, Marta | pt |
dc.contributor.author | Correia, António | pt |
dc.contributor.author | Henriques, Isabel | pt |
dc.date.accessioned | 2013-10-07T16:08:51Z | - |
dc.date.available | 2013-10-07T16:08:51Z | - |
dc.date.issued | 2012-06 | - |
dc.identifier.issn | 0099-2240 | pt |
dc.identifier.uri | http://hdl.handle.net/10773/11067 | - |
dc.description.abstract | We compared the resistomes within polluted and unpolluted rivers, focusing on extended-spectrum beta-lactamase (ESBL) genes, in particular blaCTX-M. Twelve rivers from a Portuguese hydrographic basin were sampled. Physicochemical and microbiological parameters of water quality were determined, and the results showed that 9 rivers were classified as unpolluted (UP) and that 3 were classified as polluted (P). Of the 225 cefotaxime-resistant strains isolated, 39 were identified as ESBL-producing strains, with 18 carrying a blaCTX-M gene (15 from P and 3 from UP rivers). Analysis of CTX-M nucleotide sequences showed that 17 isolates produced CTX-M from group 1 (CTX-M-1, -3, -15, and -32) and 1 CTX-M that belonged to group 9 (CTX-M-14). A genetic environment study revealed the presence of different genetic elements previously described for clinical strains. ISEcp1 was found in the upstream regions of all isolates examined. Culture-independent blaCTX-M-like libraries were comprised of 16 CTX-M gene variants, with 14 types in the P library and 4 types in UP library, varying from 68% to 99% similarity between them. Besides the much lower level of diversity among CTX-M-like genes from UP sites, the majority were similar to chromosomal ESBLs such as blaRAHN-1. The results demonstrate that the occurrence and diversity of blaCTX-M genes are clearly different between polluted and unpolluted lotic ecosystems; these findings favor the hypothesis that natural environments are reservoirs of resistant bacteria and resistance genes, where anthropogenic-driven selective pressures may be contributing to the persistence and dissemination of genes usually relevant in clinical environments. | pt |
dc.language.iso | eng | pt |
dc.publisher | American Society for Microbiology | pt |
dc.relation | FCT - SFRH/BD/43468/2008 | pt |
dc.relation | FCT - SFRH/BPD/ 63487/2009 | pt |
dc.rights | openAccess | por |
dc.title | Resistance to Broad-Spectrum Antibiotics in Aquatic Systems: Anthropogenic Activities Modulate the Dissemination of blaCTX-M-Like Genes | pt |
dc.type | article | |
dc.peerreviewed | yes | pt |
ua.distribution | international | pt |
degois.publication.firstPage | 4134 | pt |
degois.publication.issue | 12 | pt |
degois.publication.issue | 12 | |
degois.publication.lastPage | 4140 | pt |
degois.publication.title | Applied and Environmental Microbiology | pt |
degois.publication.volume | 78 | pt |
dc.identifier.doi | 10.1128/AEM.00359-12 | pt |
Appears in Collections: | CESAM - Artigos DBio - Artigos Ria de Aveiro - Artigos |
Files in This Item:
File | Description | Size | Format | |
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5_Resistance to Broad-Spectrum.pdf | 583.86 kB | Adobe PDF | View/Open |
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