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 Simple and solvent-free methodology for simultaneous quantification of methanol and acetic acid content of plant polysaccharides based on headspace solid phase microextraction-gas chromatography (HS-SPME-GC-FID)
Please use this identifier to cite or link to this item http://hdl.handle.net/10773/6969

title: Simple and solvent-free methodology for simultaneous quantification of methanol and acetic acid content of plant polysaccharides based on headspace solid phase microextraction-gas chromatography (HS-SPME-GC-FID)
authors: Nunes, Cláudia
Rocha, Sílvia M.
Saraiva, Jorge
Coimbra, Manuel A.
keywords: Methanol
Acetic acid
Solid phase microextraction
Headspace analysis
Plant polysaccharides
issue date: 2006
publisher: Elsevier
abstract: A simple and solvent-free methodology is proposed for the simultaneous determination of methanol and acetic acid content of any type of plant cell wall polysaccharides. The methanol and acetic acid, released by saponification, are extracted from the headspace of the aqueous solution by solid phase microextraction (HS-SPME) using a DVB/Carboxen/PDMS fibre. The analytes are separated by gas chromatography and detected using a flame ionization detector (GC-FID). The quantification of methanol and acetic acid is done using external calibration curves. A linear relationship between the concentration of methanol (40–100 mg L−1) and acetic acid (25–105 mg L−1) and their GC peak area was observed (r2=0.987 and 0.988, respectively) with a reproducibility of 10%. HS-SPME-GC-FID revealed to be a clean, simple, fast and reliable methodology for the determination of the methanol and acetic acid content of cell wall polysaccharide extracts.
URI: http://hdl.handle.net/10773/6969
ISSN: 0144-8617
publisher version/DOI: http://dx.doi.org/10.1016/j.carbpol.2005.11.039
source: Carbohydrate Polymers
appears in collectionsQOPNA - Artigos

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