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 High pressure pre-treatments promote higher rate and degree of enzymatic hydrolysis of cellulose
Please use this identifier to cite or link to this item http://hdl.handle.net/10773/6945

title: High pressure pre-treatments promote higher rate and degree of enzymatic hydrolysis of cellulose
authors: Ferreira, Ana R. F. C.
Figueiredo, Andreia B.
Evtuguin, Dmitry V.
Saraiva, Jorge A.
keywords: High pressure pre-treatments
cellulose hydrolysis
Bioethanol
issue date: 8-Aug-2011
publisher: Royal Society of Chemistry
abstract: The effect of high pressure (HP) pre-treatments on the subsequent enzymatic hydrolysis of cellulose from bleached kraft Eucalyptus globulus pulp by cellulase from Tricoderma viride was evaluated. Pressure pre-treatments of 300 and 400 MPa during 5–45 min, lead to both an increased rate and degree of hydrolysis, reaching values ranging from 1.5- to 1.9-fold, quantified by the formation of reducing sugars. Both the pressure and time under pressure influenced the enzymatic hydrosability of the cellulosic pulps, with the former being more important. The results indicate that the pressure pre-treatments promoted an increased accessibility of cellulose towards cellulase in the cell wall. The results obtained open promising possibilities, to contribute to overcome conventional limitations of enzymatic cellulose hydrolysis for the production of fermentable glucose, for the production of second generation bioethanol and chemicals by enhancement of both rate and yield of hydrolysis. The results are also of interest for the preparation of “pressure engineered” celullose with incremented tailored hydrolysis patterns.
URI: http://hdl.handle.net/10773/6945
ISSN: 1463-9262
publisher version/DOI: http://dx.doi.org/10.1039/C1GC15500H
source: Green Chemistry
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