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 Development of a microarray for Enchytraeus Albidus (oligochaeta): preliminary tool with diverse applications
Please use this identifier to cite or link to this item http://hdl.handle.net/10773/6612

title: Development of a microarray for Enchytraeus Albidus (oligochaeta): preliminary tool with diverse applications
authors: Amorim, MJB
Novais, SC
Van Der Ven, K
Vandenbrouck, T
Soares, AMVM
De Coen, W
keywords: cDNA library development
Stress-induced genes
Hazard assessment
DNA microarray
Enchytraeus albidus
issue date: 2011
publisher: SETAC Press
abstract: Standard bioassays allow hazard assessment at the population level, but much remains to be learned about the molecular level response of organisms to stressors. The main aim of this study was the development of a DNA microarray for Enchytraeus albidus, a common soil worm species. Further, this microarray was tested using worms exposed to Cu, phenmedipham, and different soil types. Hybridization onto the developed microarray revealed several genes with homology to known sequences. Genes of interest were confirmed through real-time polymerase chain reaction. It was possible to discriminate between natural and chemical stressors and chemical concentrations. Gene responses were detected under conditions known to have effects in the reproduction of individuals. It was confirmed that the integration of different endpoints improves the assessment process and enhances the understanding of the modes of action of stressors. The chemical stress-induced genes were related to factors such as immune response, stress response, metabolic processes, and/or signal transduction. The present study represents the first step of a gene-level study in the ecologically relevant and standard test species E. albidus. It demonstrates the usefulness of cDNA normalization in the production of cDNA libraries of ecotoxicological standard organisms that are not genome models like E. albidus. Environ. Toxicol. Chem. 2011;30:1395-1402. (C) 2011 SETAC
URI: http://hdl.handle.net/10773/6612
ISSN: 0730-7268
publisher version/DOI: http://dx.doi.org/10.1002/etc.512
source: Environmental Toxicology and Chemistry
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