DSpace
 
  Repositório Institucional da Universidade de Aveiro > Departamento de Biologia > BIO - Artigos >
 Flow cytometric assessment of Cd genotoxicity in three plants with different metal accumulation and detoxification capacities
Please use this identifier to cite or link to this item http://hdl.handle.net/10773/6372

title: Flow cytometric assessment of Cd genotoxicity in three plants with different metal accumulation and detoxification capacities
authors: Monteiro, MS
Rodriguez, E
Loureiro, J
Mann, RM
Soares, AMVM
Santos, C
keywords: DNA
Lactuca sativa
Metal
Thlaspi arvense
Thlaspi caerulescens
issue date: 2010
publisher: Elsevier
abstract: Cadmium (Cd) is a widespread environmental contaminant, strongly mutagenic and known to cause DNA damage in plants. In this work, flow cytometry (FCM) was applied to determine if in vivo exposure to Cd would induce genotoxic effects at the genome level. The hyper-accumulator Thlaspi caerulescens J. & C. Presl), the related non-accumulator Thlaspi arvense L. and the accumulator crop species Lactuca sativa L. were germinated in distilled water and grown in modified Hoagland's medium with increasing concentrations of Cd(NO(3))(2) (0, 1, 10 and 100 mu M). After 28 days of exposure, shoot and root growth was recorded and the tissues were harvested for Cd and FCM analysis. In general, roots from treated plants contained higher content of Cd than leaves and growth inhibition was observed in the treated plants. Nuclear DNA content was estimated and the G(0)/G(1) full peak coefficient of variation (FPCV), as an indicator of clastogenic damage, was recorded. In T. arvense and T. caerulescens no significant differences were detected between control and exposed plants. Leaves of L sativa exposed to 10 mu M Cd presented a statistically significant increase in FPCV values in comparison with the control group. Furthermore, roots exposed to 100 mu M Cd presented a reduction in nuclear DNA content and an increase in FPCV when compared to the control. FCM data indicates that no major DNA damage was induced on both Cd-exposed Thlaspi species and L. sativa leaves. On the contrary, results obtained with L. sativa roots suggests clastogenic damage in these organs exposed to 100 mu M of Cd. (C) 2010 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/10773/6372
ISSN: 0147-6513
publisher version/DOI: http://dx.doi.org/10.1016/j.ecoenv.2010.06.020
source: Ecotoxicology and Environmental Safety
appears in collectionsBIO - Artigos

files in this item

file description sizeformat
216_EES_2010_73_1231.pdf467.19 kBAdobe PDFview/open
Restrict Access. You can Request a copy!
statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! RCAAP OpenAIRE DeGóis
ria-repositorio@ua.pt - Copyright ©   Universidade de Aveiro - RIA Statistics - Powered by MIT's DSpace software, Version 1.6.2