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|title: ||Analysis of Non-Aromatic Organic Acids in Beer by CE and Direct Detection Mode with Diode Array Detection|
|authors: ||Erny, Guillaume L.|
Rodrigues, Joao E. A.
Gil, Ana M.
Barros, Antonio S.
Esteves, Valdemar I.
|keywords: ||Capillary electrophoresis|
Diode array detection
Organic acids in beer
|issue date: ||2009|
|publisher: ||Springer Verlag|
|abstract: ||A method for the analysis of the main non-aromatic organic acids in beer using capillary
electrophoresis is presented. In this work, malic, citric, succinic, pyruvic, acetic and lactic
acids are separated using a sodium hydrogen phosphate background electrolyte with direct
detection mode with a diode array detector. The separation exhibits lower sensitivity than
equivalent methods with indirect detection mode, however, the risk of co-migration with
unknown compounds in beer matrixes is significantly reduced. This is due to (i) a higher
efficiency (250,000–400,000 theoretical plates), (ii) a higher selectivity than any equivalent
method using an indirect detection mode, and (iii) the possibility to monitor other wavelengths
in parallel (260 nm for example) to check for possible co-migration with phenolic or
benzoic acids. This was critical when working with beer samples as an unknown compound
absorbing at 200 and 260 nm was detected in the neighbourhood of malic, citric and
succinic acids. Such co-migration will not have been detected using single wavelength
detection below 200 nm or indirect detection mode.|
|publisher version/DOI: ||http://dx.doi.org/10.1365/s10337-009-1377-4|
|appears in collections||DQ - Artigos|
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