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 Application of RAPD DNA fingerprinting in taxonomic identification of amphipods: a case-study with Gammarus species (Crustacea : Amphipoda)
Please use this identifier to cite or link to this item http://hdl.handle.net/10773/5082

title: Application of RAPD DNA fingerprinting in taxonomic identification of amphipods: a case-study with Gammarus species (Crustacea : Amphipoda)
authors: Costa, Filipe O.
Cunha, Marina R.
Neuparth, Teresa
Theodorakis, Christopher W.
Costa, M. Helena
Shugart, Lee R.
keywords: polymorphic DNA (RAPD) fingerprinting
issue date: Feb-2004
publisher: Cambridge University Press
abstract: In this study randomly amplified polymorphic DNA (RAPD) fingerprinting is proposed for species identification of Gammarus, based on the electrophorestic position of amplified DNA bands. Three common marine amphipods of European estuaries-G. chevreuxi, G insensibilis and G. locusta were profiled using ten RAPD primers, accompanied by a careful morphological identification. Nine of these primers produced a very distinct species-specific RAPD profile, allowing unambiguous differentiation of amphipod species assayed. The RAPD fingerprints were here characterized by 8-12 amplicons for each primer. Each amplicon was visualized as a band of known molecular length, with characteristic band thickness mid density. A total of 78 diagnostic bands, based on the most robust and evident amplicons found for each primer and species, are proposed for identification of the Gammarus species analysed. These results allowed us to identify an unknown amphipod species from a previous study as G. insensibilis, only based on the RAPD fingerprint. One primer was sufficient for this identification. A taxonomic identification system integrating molecular and morphological tools is proposed for Gammarus.
URI: http://hdl.handle.net/10773/5082
ISSN: 0025-3154
publisher version/DOI: http://dx.doi.org/10.1017/S0025315404009038h
source: Journal of the Marine Biological Association of the UK
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