Please use this identifier to cite or link to this item
|title: ||Automated high-throughput screening of carbon nanotube-based bio-nanocomposites for bone cement applications|
|authors: ||Gonçalves, Paula P.|
Singh, Manoj K.
Silva, Virgília S.
LeDuc, Philip R.
Marques, Paula A. A. P.
Sousa, António C. M.
|keywords: ||bone cement|
|issue date: ||Nov-2011|
|publisher: ||International Union of Pure & Applied Chemistry|
|abstract: ||In this work we demonstrate the potential of using an automated cell viability analyzer
for developing high-throughput screening of orthopedic bioactive materials. We used a
biomaterial of carbon nanotubes (CNTs)-based composite integrated with hydroxyapatite/
polymethyl methacrylate (HA/PMMA) with controlled physical and chemical properties to
evaluate the usefulness of morphometric analysis in conjunction with trypan blue dye exclusion
assays in MG63 cell cultures. The MG63 cell line, derived from human bone osteo -
sarcoma, is often used as a model for studying osteoblast-like cellular response to bioactive
materials for orthopedic surgery. The viability analyzer, Vi-CELLTM XR, Beckman Coulter,
was used with trypan blue dye exclusion method in cell suspensions obtained after
trypsinization along with determining the distribution plots of cell diameter and circularity,
which are critical cellular characteristics. In addition, the activity of alkaline phosphatase
(ALP), a typical representation of osteogenic activity of osteoblasts, was also measured
spectrophotometrically using p-nitrophenol phosphate as the substrate. Comparative analysis
of the frequency histogram of average cell diameter and circularity allowed for the analyses
of significant alterations in cell morphology not only over time in control cultures (spherical
vs. a flat morphology) but also with respect to PMMA and HA nanocomposites. After cell
exposure to HA/PMMA/CNTs, a shift toward loss of cell circularity was observed. The
appearances of more differentiated morphologic features were well correlated with the
increase of secreted ALP activity. In conclusion, the evaluation of material-induced changes
of cell morphology could represent a valuable prescreening test for bioactive properties.|
|publisher version/DOI: ||http://dx.doi.org/10.1351/PAC-CON-11-04-06|
|source: ||Pure & Applied Chemistry|
|appears in collections||CESAM - Artigos|
BIO - Artigos
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.