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http://hdl.handle.net/10773/37225
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Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Gaaied, Sonia | pt_PT |
dc.contributor.author | Oliveira, Miguel | pt_PT |
dc.contributor.author | Le Bihanic, Florane | pt_PT |
dc.contributor.author | Cachot, Jérôme | pt_PT |
dc.contributor.author | Banni, Mohamed | pt_PT |
dc.date.accessioned | 2023-04-20T13:13:39Z | - |
dc.date.available | 2023-04-20T13:13:39Z | - |
dc.date.issued | 2019-06 | - |
dc.identifier.issn | 0045-6535 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10773/37225 | - |
dc.description.abstract | The present study aims to assess the effects of 2,4-D herbicide on biotransformation and oxidative stress status of zebrafish larvae. Animals were exposed to a range of sublethal concentrations (0.02–0.8 mg/L) and biomarkers at transcriptomic level and biochemical level were assessed. Chemical analysis with showed that the bioaccumulation of 2,4-D in 96 hpf zebrafish larvae were increased in a concentration-dependent manner. This herbicide induced significant effects at both gene expression and enzymatic activities levels after at 96 hpf. Results of mRNA expression showed a differential transcription regulation with all target genes depending on the tested concentrations. The mRNA level of gsr and cyp1a were up regulated at the highest dose of herbicide (0.8 mg/L). The gene expression of gstp1 showed an up regulation at lower dose (0.02 mg/L) and a down regulation at the highest dose (0.8 mg/L) of 2,4-D. A significant induction of EROD activity and inhibition of GST activity were noted in groups exposed to 0.8 mg/L of 2,4-D. Considering the antioxidant defenses, the activity of CAT was increased in larvae exposed to 0.8 mg/L of herbicide and GPx activity was induced at lower doses of 2,4-D (0.02 and 0.051 mg/L). Moreover, peroxidative damage, assessed as MDA content, was markedly increased in larvae exposed to high 2,4-D concentration. Overall, the present study data indicate that bioaccumulation of 2,4-D in 96 hpf zebrafish larvae and alterations in detoxification and oxidative stress related parameters, likely associated with ROS production, which may endanger the embryo-larval stages development of fish. | pt_PT |
dc.language.iso | eng | pt_PT |
dc.publisher | Elsevier | pt_PT |
dc.relation | UR13A6R08 | pt_PT |
dc.rights | openAccess | pt_PT |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
dc.subject | Herbicide | pt_PT |
dc.subject | Gene expression | pt_PT |
dc.subject | Biotransformation | pt_PT |
dc.subject | Oxidative stress | pt_PT |
dc.subject | Zebrafish | pt_PT |
dc.subject | Early life stages | pt_PT |
dc.title | Gene expression patterns and related enzymatic activities of detoxification and oxidative stress systems in zebrafish larvae exposed to the 2,4-dichlorophenoxyacetic acid herbicide | pt_PT |
dc.type | article | pt_PT |
dc.description.version | published | pt_PT |
dc.peerreviewed | yes | pt_PT |
degois.publication.firstPage | 289 | pt_PT |
degois.publication.lastPage | 297 | pt_PT |
degois.publication.title | Chemosphere | pt_PT |
degois.publication.volume | 224 | pt_PT |
dc.identifier.doi | 10.1016/j.chemosphere.2019.02.125 | pt_PT |
Aparece nas coleções: | CESAM - Artigos DBio - Artigos |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Gene expression patterns and related enzymatic activities of detoxification and.pdf | 895.84 kB | Adobe PDF | Ver/Abrir |
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