Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/20577
Title: | Human plasma stability during handling and storage: impact on NMR metabolomics |
Author: | Pinto, Joana Domingues, M. Rosario M. Galhano, Eulalia Pita, Cristina Almeida, Maria do Ceu Carreira, Isabel M. Gil, Ana M. |
Keywords: | HUMAN BLOOD H-1-NMR SERUM ANTICOAGULANT SPECTROSCOPY VALIDATION COLLECTION SPECTRA URINE |
Issue Date: | 2014 |
Publisher: | ROYAL SOC CHEMISTRY |
Abstract: | This work contributes to fill in some existing gaps in the knowledge of human plasma degradability during handling and storage, a paramount issue in Nuclear Magnetic Resonance (NMR) metabolomics. Regarding the comparison between heparin and EDTA anti-coagulant collection tubes, the former showed no interference of the polysaccharide, while conserving full spectral information. In relation to time/temperature conditions, room temperature was seen to have a large impact on lipoproteins and choline compounds from 2.5 hours. In addition, short-term storage at -20 degrees C was found suitable up to 7 days but, for periods up to 1 month, -80 degrees C was recommended. Furthermore, in the case of reusing plasma samples, no more than 3 consecutive freeze-thaw cycles were found advisable. Finally, the impact of long-term -80 degrees C storage (up to 2.5 years) was found almost negligible, as evaluated on a partially matched non-fasting cohort (n = 49), after having investigated the possible confounding nature of the particular non-fasting conditions employed. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/20577 |
DOI: | 10.1039/c3an02188b |
ISSN: | 0003-2654 |
Publisher Version: | 10.1039/c3an02188b |
Appears in Collections: | CICECO - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Human plasma stability during handling and storage impact on NMR metabolomics_10.1039c3an02188b.pdf | 804.96 kB | Adobe PDF |
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